Rezistența la antimicrobiene. Caracteristica rezistenței la preparate antimicrobiene a bacteriilor gram-negative

Victoria Bucov; Olga Burduniuc; Greta Balan; Maria Grumeza; Olga Craciun; Maria Bivol

Rezistența la antimicrobiene. Caracteristica rezistenței la preparate antimicrobiene a bacteriilor gram-negative

Autori

Victoria Bucov Agenţia Naţională pentru Sănătate Publică https://orcid.org/0009-0005-1042-5947
Olga Burduniuc Agenţia Naţională pentru Sănătate Publică https://orcid.org/0000-0002-6944-0800
Greta Balan Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu“ https://orcid.org/0000-0003-3704-3584
Maria Grumeza Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu“ https://orcid.org/0009-0007-9897-7766
Olga Craciun Agenţia Naţională pentru Sănătate Publică
Maria Bivol Agenţia Naţională pentru Sănătate Publică https://orcid.org/0000-0002-4804-7137

Cuvinte cheie:

preparate antimicrobiene, bacterii gramnegative, rezistenţă

Rezumat

Scopul articolul este reviul literaturii privind rezistența bacteriilor Gram-negative la preparate antimicrobiene. Au fost analizate 131 de surse, inclusiv documentele OMS și publicații științifice. Rezistența bacteriană rămâne o amenințare semnifi cativă și o cauză principală de deces la nivel mondial. Bacteriile rezistente proliferează prin selecție naturală atunci când cele sensibile la medicamente sunt îndepărtate de antibiotice. Aceste organisme se pot răspândi în toate unitățile de îngrijire a sănătății, precum și în comunitate, fapt ce necesită o atenție sporită la măsurile de control al infecțiilor și administrarea medicamentelor care conțin β-lactam în efortul de a controla selecția de agenți patogeni și mai nocivi. Au fost raportate mai multe strategii pentru combaterea și controlul bacteriilor Gram-negative rezistente, cum ar fi dezvoltarea agenților auxiliari antimicrobieni, modifi carea structurală a antibioticelor existente, cercetarea și studiul structurilor chimice cu noi mecanisme de acțiune. Pe lângă terapia nouă, un sistem efi cient de control al infecțiilor, alături de utilizarea prudentă a preparatelor antimicrobiene, este extrem de important.

Referințe

1. Hua M., Guo J., Li M. et al. A Dual-Replicon Shuttle Vector System for Heterologous Gene Expression in a Broad Range of Gram-Positive and Gram-Negative Bacteria. In: Curr. Microbiol. 2018 Oct; 75(10): 1391-1400. https://doi.org/10.1007/s00284-018-1535-8

2. Subramaniam G., Girish M. Antibiotic Resistance - A Cause for Reemergence of Infections. In: Indian J. Pediatr. 2020 Feb 5. https://doi.org/10.1007/s12098-019-03180-3

3. Marylise Duperthuy. Antimicrobial Peptides: Virulence and Resistance Modulation in Gram-Negative Bacteria. In: Microorganisms. 2020 Feb; 8(2): 280. https://doi.org/10.3390/microorganisms8020280

4. Buitinck S., Jansen R., Rijkenberg S. The ecological eff ects of selective decontamination of the digestive tract (SDD) on antimicrobial resistance: a 21-year longitudinal single-centre study. In: Crit. Care. 2019 Jun 7; 23(1): 208. https://doi.org/10.1186/s13054-019-2480-z

5. Butler M.S., Blaskovich M.A., Owen J.G. et al. Old dogs and new tricks in antimicrobial discovery. In: Curr. Opin. Microbiol. 2016 Oct; 33: 25-34. https://doi.org/10.1016/j.mib.2016.05.011

6. Gasu E.N., Ahor H.S., Borquaye L.S. Peptide Extract from Olivancillaria hiatula Exhibits Broad-Spectrum Antibacterial Activity. In: Biomed Res. Int. 2018 Dec 23; 2018: 6010572. https://doi.org/10.1155/2018/6010572

7. Wei H.Y., Tan B.Y., Yang L.J. et al. Antibiotic resistance of gram-negative Bacillis isolated from children with bronchopneumonia. In: Zhongguo dang dai er ke za zhi. 2011 Jan; 13(1): 20-2.

8. Jamil B., Habib H., Abbasi S. et al. Cefazolin loaded chitosan nanoparticles to cure multi drug resistant Gram-negative pathogens. In: Carbohydr. Polym. 2016 Jan 20; 136: 682-91. https://doi.org/10.1016/j.carbpol.2015.09.078

9. Gunzer F., Rudolph W.W., Bunk B. et al. Whole-genome sequencing of a large collection of Myroides odoratimimus and Myroides odoratus isolates and antimicrobial susceptibility studies. In: Emerg. Microbes Infect. 2018 Apr 4;7(1): 61. 10. Koiava T., Gonçalves D., Palmeira J. et al. Detection of https://doi.org/10.1038/s41426-018-0061-x

CTX-M gene antibiotics rezistance in Clesiella pneumonia isolates of hospitals in Adjaria (Georgia). In: Georgian Med. News. 2016 Sep; (258): 50-54.

11. Lee J.H., Takahashi M., Jeon J.H. et al. Dual activity of PNGM-1 pinpoints the evolutionary origin of subclass B3 metallo-β-lactamases: a molecular and evolutionary study. In: Emerg. Microbes Infect. 2019; 8(1):1688-1700. https://doi.org/10.1080/22221751.2019.1692638

12. Poirel L., Madec J.Y., Lupo A. et al. Antimicrobial Resistance in Escherichia coli. In: Microbiol. Spectr. 2018 Jul; 6(4). https://doi.org/10.1128/microbiolspec.ARBA-0026-2017

13. Bush K., Bradford P.A. Epidemiology of β-LactamaseProducing Pathogens. In: Microbiol. Rev. 2020 Feb 26; 33(2). pii: e00047-19. https://doi.org/10.1128/CMR.00047-19

14. Kim Y.H. Conditional probability analysis of multidrug resistance in Gram-negative bacilli isolated from tertiary medical institutions in South Korea during 1999-2009.In: J. Microbiol. 2016 Jan; 54(1): 50-56. https://doi.org/10.1007/s12275-016-5579-9

15. Arsalan A., Naqvi S.B., Sabah A. et al. Resistance pattern of clinical isolates involved in surgical site infections. In: Pak. J. Pharm. Sci. 2014 Jan; 27(1): 97-102.

16. Al Wutayd O., Al Nafeesah A., Adam I. The antibiotic susceptibility patterns of uropathogens isolated in Qassim, Saudi rabia. In: J. Infect. Dev. Ctries. 2018 Nov 30; 12(11): 946-952. https://doi.org/10.3855/jidc.10553

17. Masseron A., Poirel L., Jamil Ali B. et al. Molecular characterization of multidrug-resistance in Gramnegative bacteria from the Peshawar teaching hospital, Pakistan. In: New Microbes New Infect. 2019 Oct 11; 32: 100605. https://doi.org/10.1016/j.nmni.2019.100605

18. Angers-Loustau A., Petrillo M. Bengtsson-Palme J. et al. The challenges of designing a benchmark strategy for bioinformatics pipelines in the identifi cation of antimicrobial resistance determinants using next generation sequencing technologies. In: Comm J-459. doi: 10.12688/f1000research.14509.2. eCollection 2018. https://doi.org/10.12688/f1000research.14509.2

19. Wang J.C.,Chi S.W., Yang T.H. et al. Label-Free Monitoring of Microorganisms and Their Responses to Antibiotics Based on Self-Powered. Microbead Sensors. In: ACS Sens. 2018, 3, 10, 2182-2190. https://doi.org/10.1021/acssensors.8b00790

20. Liang T., Leung L.M., Opene B. et al. Rapid Microbial Identifi cation and Antibiotic Resistance Detection by Mass Spectrometric Analysis of Membrane Lipids. In: Anal. Chem. 2019 Jan 15; 91(2): 1286-1294. https://doi.org/10.1021/acs.analchem.8b02611

21. Rao R. P., Sharma S., Mehrotra T. et al. Rapid Electrochemical Monitoring of Bacterial Respiration for Gram-Positive and Gram-Negative Microbes: Potential Application in Antimicrobial Susceptibility Testing. In: Anal. Chem. 2020 Mar 17; 92(6): 4266-4274. https://doi.org/10.1021/acs.analchem.9b04810

22. Cansizoglu M.F., Tamer Y.T., Farid M. et al. Rapid ultrasensitive detection platform for antimicrobial susceptibility testing. In: PLoS Biol. 2019 May 30; 17(5): e3000291. https://doi.org/10.1371/journal.pbio.3000291

23. Sinkó J. Clinical aspects of severe infections caused by antibiotic-resistant Gram-negative bacteria. The Empire strikes back? In: Orv. Hetil. 2017 Oct; 158(39): 1528-1534.

24. Wyres K.L., Holt K.E. Klebsiella pneumoniae as a key trafficker of drug resistance genes from environmental to clinically important bacteria. In: Curr. Opin. Microbiol. 2018 Oct; 45: 131-139. https://doi.org/10.1016/j.mib.2018.04.004

25. Li B., Qiu Y., Glidle A. et al. Single cell growth rate and morphological dynamics revealing an "opportunistic" persistence. In: Analyst. 2014 Jul 7; 139(13): 3305-13. https://doi.org/10.1039/C4AN00170B

26. Shelburne S.A., Kim J., Munita J.M. et al. WholeGenome Sequencing Accurately Identifi es Resistance to Extended-Spectrum β-Lactams for Major GramNegative Bacterial Pathogens. In: Clin. Infect. Dis. 2017 Sep 1; 65(5): 738-745. https://doi.org/10.1093/cid/cix417

27. Burckhardt R.M., Escalante-Semerena J.C. In Bacillus subtilis, the SatA (Formerly YyaR) Acetyltransferase Detoxifi es Streptothricin via Lysine Acetylation. In: Appl. Environ. Microbiol. 2017 Oct 17; 83(21). pii: e01590-17. https://doi.org/10.1128/AEM.01590-17

28. Watkins R.R., Papp-Wallace K.M., Drawz S.M. et al. Novel β-lactamase inhibitors: a therapeutic hope against the scourge of multidrug resistance. In: Front. Microbiol. 2013 Dec 24; 4: 392. https://doi.org/10.3389/fmicb.2013.00392

29. Ginovyan M., Keryan A., Bazukyan I. et al. The large scale antibacterial, antifungal and anti-phage effi - ciency of Petamcin-A: new multicomponent preparation for skin diseases treatment. In: Ann ClinMicrobiol Antimicrob. 2015 May 18; 14: 28. https://doi.org/10.1186/s12941-015-0087-z

30. Sanyasi S., Majhi R.K., Kumar S. et al. Polysaccharidecapped silver Nanoparticles inhibit biofi lm formation and eliminate multi-drug-resistant bacteria by disrupting bacterial cytoskeleton with reduced cytotoxicity towards mammalian cells. In: Sci. Rep. 2016 Apr 29; 6: 24929. https://doi.org/10.1038/srep24929

31. Lin Y.M., Ghosh M., Miller P.A. et al. Synthetic sideromycins (skepticism and optimism): selective generation of either broad or narrow spectrum Gram-negative antibiotics. In: Biometals. 2019 Jun; 32(3): 425-451. https://doi.org/10.1007/s10534-019-00192-6

32. Ahmed R.N., Daniel F., Gbala I.D., Sanni A. Potentials of Actinomycetes from Reserved Environments as Antibacterial Agents Against Drug-Resistant Clinical Bacterial Strains. In: Ethiop. J. Health Sci. 2020 Mar; 30 (2): 251-258. https://doi.org/10.4314/ejhs.v30i2.13

33. Bakht J., Iftikhar Z., Shafi M. et al. Report - Screening of medicinally important Berberis lyceum for their antimicrobial activity by disc diff usion assay. In: Pak. J. Pharm. Sci. 2017 Sep; 30 (5): 1783-1789.

34. Mohamed N.A., Fahmy M.M. Synthesis and antimicrobial activity of some novel cross-linked chitosan hydrogels. In: J. Mol. Sci. 2012; 13(9): 11194-209. https://doi.org/10.3390/ijms130911194

35. Kim W.H., Lillehoj H.S., Gay C.G. Using genomics to identify novel antimicrobials. In: Sci. Tech. 2016 Apr; 35(1): 95-103. https://doi.org/10.20506/rst.35.1.2420

36. Nobakht M., Trueman S.J., Wallace H.M. et al. Antibacterial Properties of Flavonoids from Kino of the Eucalypt Tree, Corymbia torelliana. In: Plants Basel. 2017 Sep 14; 6(3). pii: E39. https://doi.org/10.3390/plants6030039

37. Saika Tariq, Saira Wani, Waseem Rasool et al. Comprehensive Review of the Antibacterial, Antifungal and Antiviral Potential of Essential Oils and Their Chemical Constituents Against Drug-Resistant Microbial Pathogens. In: PMID: 31195112. https://doi.org/10.1016/j.micpath.2019.103580

38. Ageitos J.M., Sánchez-Pérez A., Calo-Mata P. et al. Antimicrobial peptides (AMPs): Ancient compounds that represent novel weapons in the fi ght against bacteria. In: Biochem Pharmacol. 2017 Jun 1; 133: 117-138. https://doi.org/10.1016/j.bcp.2016.09.018

39. Baltzer S.A., Brown M.H. Antimicrobial peptides: promising alternatives to conventional antibiotics. In: J. Mol. Microbiol. Biotechnol. 2011; 20(4): 228-35. https://doi.org/10.1159/000331009

40. Deng W., Fu T., Zhang Z. et al. L-lysine potentiates aminoglycosides against Acinetobacter baumannii via regulation of proton motive force and antibiotics uptake. In: Emerg. Microbes Infect. 2020 Mar 20; 9(1): 639-650. https://doi.org/10.1080/22221751.2020.1740611

41. Breijyeh Z., Jubeh B., Karaman R. Resistance of GramNegative Bacteria to Current Antibacterial Agents and Approaches to Resolve In: It. Molecules. 2020 Mar 16; 25(6). pii: E1340. https://doi.org/10.3390/molecules25061340

42. El Malah T., Nour H.F., Satti A.E. et al. Design, Synthesis, and Antimicrobial Activities of 1,2,3-Triazole Glycoside Clickamers. In: Molecules. 2020 Feb 12; 25(4). pii: E790. https://doi.org/10.3390/molecules25040790

43. Nichols D., Pimentel M.B., Borges F.T.P. et al. Sustained Release of Phosphates From Hydrogel Nanoparticles Suppresses Bacterial Collagenase and Biofi lm Formation in vitro. In: Front Bioeng Biotechnol. 2019 Jun 26; 7: 153. https://doi.org/10.3389/fbioe.2019.00153

Descărcări

Publicat

17.06.2026

Număr

Nr. 1(88) (2021)

Secțiune

Articole

Licență

Această lucrare este licențiată în temeiul Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Cum cităm

[1]

Bucov, V. et al. 2026. Rezistența la antimicrobiene. Caracteristica rezistenței la preparate antimicrobiene a bacteriilor gram-negative. Public Health Economy and Management in Medicine. 1(88) (Jun. 2026), 50–56.