Obtaining and determining antioxidant activity of polyphenolic extracts obtained from propolis
Keywords:
propolis, soft extract, polyphenols, antioxidant activityAbstract
Over the years, time has given us a whole series of novelties in the fi eld of natural medicine, which have managed to truly prove their value and keep up to date. Th e application of apitherapy has been known since ancient times and today the interest in this branch of natural medicine is growing worldwide. Among bee products, with an indisputable value is also propolis, which is currently widely studied, and as a result is the subject of multiple therapeutic uses based on evidence. Research in the fi eld of propolis preparations has expanded considerably and is of major medical and pharmaceutical interest. Propolis is an excellent anti-infl ammatory, healing and epithelializing, hepatoprotective, immuno-biostimulator, capillary tonic and tissue regenerator. It is remarkable that, due to its complexity and compositional variability, propolis retains its full antimicrobial capacity, unlike synthetic antibiotics, which bacteria develop resistance over time, requiring the periodic introduction of new antibiotics. Th e study on obtaining and analyzing propolis extracts, by determining the total polyphenolic and antioxidant activity, by scavenging method of the free radical DPPH, is an important premise for the use and development of new pharmaceutical forms containing propolis for multiple curative-prophylactic uses based on local raw materials.
References
1. Ahn M., Kumazawa S., Usui Y. et al. Antioxidant activity and constituents of propolis collected in various areas of China. Food Chemistry. 2007, vol. 101, pp. 1383-1392. https://doi.org/10.1016/j.foodchem.2006.03.045
2. Bankova V. Chemical diversity of propolis and the problem of standardization. Journal of Ethnopharmacology. 2005, vol. 100, pp. 114-117. https://doi.org/10.1016/j.jep.2005.05.004
3. Brand-Williams W. Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology. 1995, vol. 28, nr. 1, pp. 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
4. Castaldo S., Capasso F. Propolis, an old remedy used in modern medicine. Fitoterapia. 2002, vol. 73 (Suppl. S1), pp. S1-S6. https://doi.org/10.1016/S0367-326X(02)00185-5
5. Dărăban A., Olah N., Burtescu R., Pripon F., Hanganu D. et al. The evaluation of antioxidant capacity of propolis originating from western Romania. Farmacia. 2019, vol. 67, pp. 111-116. https://doi.org/10.31925/farmacia.2019.1.15
6. European Pharmacopoeia. Strasbourg, 2014, vol. 1, p. 806.
7. Kurek-Gorecka A., Rzepecka-Stojko A., Gorecki M. et al. Structure and antioxidant activity of polyphenols derived from propolis. Molecules. 2014, vol. 19, pp. 78-101. https://doi.org/10.3390/molecules19010078
8. Laura C., Marco B., Jianbo X. et al. Therapeutic properties of bioactive compounds from different honeybee products. Frontiers in Pharmacology. 2017. https://doi.org/10.3389/fphar.2017.00412
9. Magalhaes L., Segundo M., Reis S. et al. Methodological aspects about in vitro evaluation of antioxidant properties. Analytica Chimica Acta. 2008, vol. 613, pp. 1-19. https://doi.org/10.1016/j.aca.2008.02.047
10. Milardovic S., Ivekovic D., Grabaric B. et al. Use of DPPH/DPPH redox couple for biamperometric determination of antioxidant activity. Electroanalysis. 2005, vol. 17, pp. 1847-1853. https://doi.org/10.1002/elan.200503312
11. Noushin A., Alireza N. Propolis diterpenes as a remarkable bio-source for drug discovery development: A review. International Journal of Molecular Sciences. 2017, vol. 18, art. 1290. https://doi.org/10.3390/ijms18061290
12. Ramanauskiene K., Inkeniene A., Petrikaite V., Briedis V. Total phenolic content and antimicrobial activity of different Lithuanian propolis solutions. Evidence-Based Complementary and Alternative Medicine. 2013, art. 842985. https://doi.org/10.1155/2013/842985
13. Ristivojevic P., Trifkovic J., Andrić F., Milojković-Opsenica D. Poplar-type propolis: chemical composition, botanical origin and biological activity. Natural Product Communications. 2015, vol. 10, nr. 11, pp. 1869-1876. https://doi.org/10.1177/1934578X1501001117
14. Shuai H., Cui-Ping Z., Kai W. et al. Recent advances in the chemical composition of propolis. Molecules. 2014, vol. 19, pp. 19610-19632.
https://doi.org/10.3390/molecules191219610
15. Vasiliki L., Prasianaki D., Krysta F. Antioxidant properties and phenolic composition of Greek propolis extracts. International Journal of Food Properties. 2014, vol. 17, nr. 3, pp. 511-522. https://doi.org/10.1080/10942912.2012.654561
16. Xue W., Karuppasamy S., Yizhe C. et al. Relationship between total phenolic contents and biological properties of propolis from 20 different regions in South Korea. BMC Complementary and Alternative Medicine. 2016.
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