Modificarea unor parametri biochimici ai culturilor bacteriene sub influența compușilor chimici și a extractelor de spirulină
DOI:
https://doi.org/10.52556/2587-3873.2025.1(103).06Cuvinte cheie:
compuşi chimici, extracte de spirulină, Bacterii, parametri biochimiciRezumat
Stabilirea particularităților de acțiune ale compușilor antimicrobieni reprezintă o prioritate atât din punctul de vedere al aprecierii efectelor curative, cât și din perspectiva promovării produsului farmaceutic de la idee până la medicamentul implementat în practica terapeutică. A fost realizat un studiu amplu, care a avut ca obiect de studiu trei compuși chimici nou-sintetizați și două extracte de spirulină. Pentru a studia modificarea parametrilor biochimici sub influența acestor compuși, au fost utilizate trei tulpini microbiene. Toate rezultatele experimentale obținute au fost supuse analizei statistice uzuale, cu aplicarea instrumentelor de statistică descriptivă și statistică inferențială. Extractele de spirulină nu au un efect toxic direct asupra tulpinilor de P. aeruginosa, E. coli, S. aureus, fapt confirmat prin menținerea nivelului de malondialdehidă și lactat-dehidrogenază eliberate la nivelul controlului. Compușii chimici aplicați în concentrații minime inhibitorii produc efecte toxice pronunțate în culturile bacteriene studiate: creșterea cantității de malondialdehidă; creșterea activității lactat-dehidrogenazei eliberate; scăderea activității enzimelor antioxidante primare. Acțiunea combinată a compușilor asupra parametrilor investigați este mai eficientă, deoarece în acest caz concentrația minimă inhibitorie a compușilor este de 2-4 ori mai mică în comparație cu cea a compușilor utilizați separat. Extractele de spirulină nu au exercitat un efect toxic asupra tulpinilor bacteriene, spre deosebire de compușii chimici care, în concentrații minime inhibitorii, au produs efecte toxice pronunțate. Atunci când am combinat compușii chimici cu cei biologici, am observat un efect sinergic al acestora.
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